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Wollaston prism-based structured illumination microscope with tunable frequency
Journal article   Open access   Peer reviewed

Wollaston prism-based structured illumination microscope with tunable frequency

Ana Doblas, Sebastian Bedoya and Chrysanthe Preza
Applied optics (2004), Vol.58(7), pp.B1-B8
03/01/2019
PMID: 30874230

Abstract

Optics Physical Sciences Science & Technology
This work proposes an alternative structured illumination (SI) system based on a Wollaston prism (WP) illuminated by the diffracted field of an incoherent linear source. The proposed WP-based SI system presents several advantages. First, the generated fringes can be approximated by a pure sinusoidal pattern; thereby, computational methods developed for sinusoidal SI can be used without the need for additional processing. Second, the SI pattern's period can be continuously varied up to the cutoff frequency of the native widefield system. Most significantly, the phase shifting of the SI pattern required for demodulation in SI microscopy can be easily accomplished using a de Senarmont compensator, which provides accurate lateral displacement of the fringes independently of the lateral modulation frequency. Experimental verifications confirm the presented theoretical predictions. (C) 2019 Optical Society of America
url
https://doi.org/10.1364/AO.58.0000B1View
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