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Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector
Journal article   Open access   Peer reviewed

Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector

Emilio Sánchez-Ortiga, Colin J R Sheppard, Genaro Saavedra, Manuel Martínez-Corral, Ana Doblas and Arnau Calatayud
Optics letters, Vol.37(7), pp.1280-1282
04/01/2012
PMID: 22466221

Abstract

Algorithms Equipment Design Image Processing, Computer-Assisted Microscopy, Confocal - instrumentation Microscopy, Confocal - methods Onions - ultrastructure Subtraction Technique
We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning.

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