Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

Ana Doblas; Ananya Dutta; Genaro Saavedra; Chrysanthe Preza

doi:10.1117/12.2290528

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Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

Ana Doblas, Ananya Dutta, Genaro Saavedra and Chrysanthe Preza

THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXV, Vol.10499, pp.104990F-104990F-7

Proceedings of SPIE

01/01/2018

DOI: https://doi.org/10.1117/12.2290528

Abstract

Microscopy

Optics

Physical Sciences

Science & Technology

Technology

Previously, a wavefront encoded (WFE) imaging system implemented using a squared cubic (SQUBIC) phase mask has been verified to reduce the sensitivity of the imaging system to spherical aberration (SA). The strength of the SQUBIC phase mask and, as consequence, the performance of the WFE system are controlled by a design parameter, A. Although the higher the A-value, the more tolerant the WFE system is to SA, this is accomplished at the expense of the effective imaging resolution. In this contribution, we investigate this tradeoff in order to find an optimal A-value to balance the effect of SA and loss of resolution.

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Title: Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask
Creators: Ana Doblas - University of Memphis
Ananya Dutta - University of Memphis
Genaro Saavedra - Universitat de València
Chrysanthe Preza - University of Memphis
Contributors: T G Brown (Editor)
C J Cogswell (Editor)
T Wilson (Editor)
Publication Details: THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXV, Vol.10499, pp.104990F-104990F-7
Series: Proceedings of SPIE
Publisher: Spie-Int Soc Optical Engineering
Number of pages: 7
Language: English
Grant note: PROMETEOII/2014/072 / Generalitat Valenciana; Center for Forestry Research & Experimentation (CIEF) DPI2015-66458-C2-1-R / Ministerio de Economia y Competitividad, Spain; Spanish Government University of Memphis 1353904 / National Science Foundation (NSF DBI); National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO)
Academic Unit: Department of Electrical and Computer Engineering
Resource Type: Conference proceeding
ISBN: 1510614834; 9781510614833; 9781510614840; 1510614842
DOI: https://doi.org/10.1117/12.2290528
Record Identifier: 9914471268701301

Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask

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