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SI-QPI-Stitcher: a user-friendly computational tool to stitch the super-resolved spectral components for accurate quantitative phase imaging (QPI) in digital holographic microscopy (DHM) with structured illumination (SI)
Conference proceeding

SI-QPI-Stitcher: a user-friendly computational tool to stitch the super-resolved spectral components for accurate quantitative phase imaging (QPI) in digital holographic microscopy (DHM) with structured illumination (SI)

S. Obando-Vasquez, A. Schneider and A. Doblas
Proceedings of SPIE, the international society for optical engineering, Vol.13604, pp.136040U-136040U-6
09/16/2025

Abstract

Structured Illumination (SI) has emerged as a powerful illumination technique for achieving super-resolution capability beyond the diffraction limit, with applications extending to Quantitative Phase Imaging (QPI) via Digital Holographic Microscopy (DHM) for label-free visualization of transparent specimens. The computational pipeline for SI-DHM holograms to generate super-resolved phase images involves several key steps, including the proper composition of the super-resolved spectrum to ensure accurate QPI measurements. This work presents a user-friendly tool to automatically weigh and compose the super-resolved spectrum, ensuring that the reconstructed image maintains quantitative phase accuracy while improving resolution.

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